Product Overview

NutriFreez® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines.  When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.  

NutriFreez® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing.  Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

·Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), ·umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC –DP)

·Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)

·Human Peripheral Blood Mononuclear Cells (PBMC’s)

·Human Endothelial Cells (EC)

·T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL’s)

·Neuron Cells

·Hybridomas

·CHO cells

·Vero Cells

Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types

NutriFreez® D10 Cryopreservation Medium Features

·Cryoprotective formulation designed to minimize dehydration effects

·Effective maintenance of cell viability, adhesion, and bioactivity

·Ready-to-use solution, simple protocol

·Suitable for use in cell banks and biopharmaceutical applications

·Animal component-free, serum-free, protein-free solution

·cGMP Manufactured